| 1. | Is further given by representing the surfaces using nurbs
给出了h d超曲面图形表示。 |
| 2. | Catagensis is marked by a loss of hydrogen , and consequently by a decrease of the hc ratio
后生阶段以氢减少必然导致h d比率降低为特征。 |
| 3. | Catagensis is marked by a loss of hydrogen , and consequently by a decrease of the h / c ratio
后生阶段以氢减少必然导致h d比率降低为特征。 |
| 4. | The rt - pcr product was inserted into puc18 and the recombinant plasmid puc18 / h d - 3 was sent to sequence ( by sagon company )
Pcr产物连于puc18质粒，重组质粒命名为puc18 h d - 3 ，送上海生工公司测序。 |
| 5. | In order to study in advanced , we need a lot of h d - 3 . in our experiments , the active recombinant fusion protein was expressed in escherichia coli system . advanced study could be continued based on our experiments
本试验利用基因工程技术，利用大肠杆菌获得具有抗菌活性的重组h d - 3融合蛋白，为进一步研究和开发h d - 3打下了基础。 |
| 6. | Extract the total rna from human tonsil and amplify the h d - 3 specific cdna sequence using rt - pcr with two special primers based on h d - 3 amino acid sequence in genbank . then the amplified product was analyzed on a 2 % agarose gel
自人扁桃体组织提取细胞总rna后，根据genbank中编码h d - 3成熟肽45个氨基酸的cdna序列，设计特异引物进行rt - pcr 。 |
| 7. | Objectives : to amplify the cdna encoding the 45 amino acid containing natural form of human p - defensin 3 and clone it into the prokaryotic expression vector . then express the recombinant protein in e . coli and purify it in order to get active recombinant h d - 3 fusion protein
目的：扩增编码h d - 3成熟肽的cdna ，将其构建于原核表达载体后利用大肠杆菌诱导表达，最终获得具有抗菌活性的h d - 3融合蛋白。 |
